Emergency services have confirmed 40 people have been injured, two of them seriously, after a local train collided with the buffers at the station of Alcala de Henares, to the east of Madrid on Friday afternoon.
The causes of the accident, which happened at around 4.00 pm, are as yet unknown, but the train driver has tested negative for alcohol and drugs and it is suspected a failure of the train's breaking system may have been at fault, Xinhua reported.
The Madrid region's Summa 112 emergency services initially reported that 45 people were treated and four suffered serious injuries from the afternoon accident, but it later lowered the total number of those seen to 39.
In a statement, the service said two were hospitalised with serious injuries, 11 more had moderate injuries and the rest were treated for bruises and anxiety.
Although the train was not travelling at speed when it hit the buffers, the Mayor of Alcala, Javier Rodriguez Palacios explained there had been a high number of injuries because a high number of passengers were standing up when the collision happened.
The injured have been taken to local hospitals, while Spanish Prime Minister, Mariano Rajoy, has sent a message of support to victims and their families, while Public Works Minister, Inigo de la Serna, has visited the scene.
A similar accident involving a commuter train that crashed into a buffer in Barcelona sent more than 50 people to the hospital in July at the height of the tourism season.
Last month, 27 people were injured after a passenger car derailed when a track flooded between the southern Spanish cities of Malaga and Seville.
(With inputs from IANS, AP)
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